rabbit polyclonal antibody bmp2-specific Search Results


rabbit polyclonal antibody bmp2-specific  (Biocompare)
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Biocompare rabbit polyclonal antibody bmp2-specific
IP-10/IL-17 co-treatment-initiated HCASMC calcification is mediated by the <t>BMP6</t> autocrine effect. a The HCASMCs were either maintained as the control or were co-treated with IP-10 and IL-17 for 3 days, after which we examined the BMP2/4/6 mRNA expression in the HCASMCs; b – d the HCASMCs were either maintained as the control or were pre-treated with IgG, BMP2-, BMP4-, or BMP6-blocking antibodies and then co-treated with IP-10 and IL-17; b HCASMC calcification was analyzed using ARS stain; c we examined the mRNA expressions OPN, OCN, and ALP using real-time PCR; d the protein expressions of OPN, OCN, and ALP were examined using the western blot test; e the HCASMCs were either maintained as the control or were treated with IP-10, IL-17, or both, for 48, 72, or 96 h, after which we examined the smad1/5 phosphorylation using the western blot test; f the HCASMCs were pretreated with control-, smad1- or smad5-specific siRNA and then either maintained as the control or co-treated with IP-10 and IL-17 for 14 days. The calcification of the HCASMCs was analyzed using ARS stain. The data in ( a – f ) are mean ± SEM from three independent experiments. * P < 0.05 vs. control cells; # P < 0.05 vs. IgG/or si-CL/IP-10 and IL-17-co-treated cells. The results in ( d – e ) are representative of three independent experiments with similar results
Rabbit Polyclonal Antibody Bmp2 Specific, supplied by Biocompare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IP-10/IL-17 co-treatment-initiated HCASMC calcification is mediated by the BMP6 autocrine effect. a The HCASMCs were either maintained as the control or were co-treated with IP-10 and IL-17 for 3 days, after which we examined the BMP2/4/6 mRNA expression in the HCASMCs; b – d the HCASMCs were either maintained as the control or were pre-treated with IgG, BMP2-, BMP4-, or BMP6-blocking antibodies and then co-treated with IP-10 and IL-17; b HCASMC calcification was analyzed using ARS stain; c we examined the mRNA expressions OPN, OCN, and ALP using real-time PCR; d the protein expressions of OPN, OCN, and ALP were examined using the western blot test; e the HCASMCs were either maintained as the control or were treated with IP-10, IL-17, or both, for 48, 72, or 96 h, after which we examined the smad1/5 phosphorylation using the western blot test; f the HCASMCs were pretreated with control-, smad1- or smad5-specific siRNA and then either maintained as the control or co-treated with IP-10 and IL-17 for 14 days. The calcification of the HCASMCs was analyzed using ARS stain. The data in ( a – f ) are mean ± SEM from three independent experiments. * P < 0.05 vs. control cells; # P < 0.05 vs. IgG/or si-CL/IP-10 and IL-17-co-treated cells. The results in ( d – e ) are representative of three independent experiments with similar results

Journal: Cell & Bioscience

Article Title: Serum IP-10 and IL-17 from Kawasaki disease patients induce calcification-related genes and proteins in human coronary artery smooth muscle cells in vitro

doi: 10.1186/s13578-020-00400-8

Figure Lengend Snippet: IP-10/IL-17 co-treatment-initiated HCASMC calcification is mediated by the BMP6 autocrine effect. a The HCASMCs were either maintained as the control or were co-treated with IP-10 and IL-17 for 3 days, after which we examined the BMP2/4/6 mRNA expression in the HCASMCs; b – d the HCASMCs were either maintained as the control or were pre-treated with IgG, BMP2-, BMP4-, or BMP6-blocking antibodies and then co-treated with IP-10 and IL-17; b HCASMC calcification was analyzed using ARS stain; c we examined the mRNA expressions OPN, OCN, and ALP using real-time PCR; d the protein expressions of OPN, OCN, and ALP were examined using the western blot test; e the HCASMCs were either maintained as the control or were treated with IP-10, IL-17, or both, for 48, 72, or 96 h, after which we examined the smad1/5 phosphorylation using the western blot test; f the HCASMCs were pretreated with control-, smad1- or smad5-specific siRNA and then either maintained as the control or co-treated with IP-10 and IL-17 for 14 days. The calcification of the HCASMCs was analyzed using ARS stain. The data in ( a – f ) are mean ± SEM from three independent experiments. * P < 0.05 vs. control cells; # P < 0.05 vs. IgG/or si-CL/IP-10 and IL-17-co-treated cells. The results in ( d – e ) are representative of three independent experiments with similar results

Article Snippet: IP-10, IL-17, and BMP6 ELISA kit, BMP2-specifc rabbit polyclonal antibody, BMP4-specific mouse monoclonal antibody, and BMP6-specific goat polyclonal antibody were come from the Biocompare (San Francisco, CA).

Techniques: Control, Expressing, Blocking Assay, Staining, Real-time Polymerase Chain Reaction, Western Blot, Phospho-proteomics

Runx2 regulates the BMP6 autocrine effect on the IP-10/IL-17 co-treatment-initiated OPN/OCN/ALP expression and calcification of HCASMCs. a The HCASMCs were either maintained as the control or were co-treated with IP-10 and IL-17 for 2, 4, 6, and 8 days; b the HCASMCs were either maintained as the control or were pre-treated with IgG or BMP6-blocking antibodies and then co-treated with IP-10 and IL-17 for 8 days; c the HCASMCs were pretreated with smad1- and smad5-specific siRNA and then either maintained as the control or co-treated with IP-10 and IL-17 for 8 days; a – c the expression of runx2 was examined using the western blot test; d – e the HCASMCs were pretreated with control- or runx2-specific siRNA and then maintained as either the control or co-treated with IP-10 and IL-17; d we examined the mRNA expressions of OPN, OCN, and ALP through real-time PCR; e the calcification of the HCASMCs was analyzed using ARS stain. The results in ( a – e ) are representative of three independent experiments with similar results. The data in ( d – e ) are mean ± SEM from three independent experiments. * P < 0.05 vs. siCL/control cells; # P < 0.05 vs. siCL/IP-10 and IL-17-co-treated cells

Journal: Cell & Bioscience

Article Title: Serum IP-10 and IL-17 from Kawasaki disease patients induce calcification-related genes and proteins in human coronary artery smooth muscle cells in vitro

doi: 10.1186/s13578-020-00400-8

Figure Lengend Snippet: Runx2 regulates the BMP6 autocrine effect on the IP-10/IL-17 co-treatment-initiated OPN/OCN/ALP expression and calcification of HCASMCs. a The HCASMCs were either maintained as the control or were co-treated with IP-10 and IL-17 for 2, 4, 6, and 8 days; b the HCASMCs were either maintained as the control or were pre-treated with IgG or BMP6-blocking antibodies and then co-treated with IP-10 and IL-17 for 8 days; c the HCASMCs were pretreated with smad1- and smad5-specific siRNA and then either maintained as the control or co-treated with IP-10 and IL-17 for 8 days; a – c the expression of runx2 was examined using the western blot test; d – e the HCASMCs were pretreated with control- or runx2-specific siRNA and then maintained as either the control or co-treated with IP-10 and IL-17; d we examined the mRNA expressions of OPN, OCN, and ALP through real-time PCR; e the calcification of the HCASMCs was analyzed using ARS stain. The results in ( a – e ) are representative of three independent experiments with similar results. The data in ( d – e ) are mean ± SEM from three independent experiments. * P < 0.05 vs. siCL/control cells; # P < 0.05 vs. siCL/IP-10 and IL-17-co-treated cells

Article Snippet: IP-10, IL-17, and BMP6 ELISA kit, BMP2-specifc rabbit polyclonal antibody, BMP4-specific mouse monoclonal antibody, and BMP6-specific goat polyclonal antibody were come from the Biocompare (San Francisco, CA).

Techniques: Expressing, Control, Blocking Assay, Western Blot, Real-time Polymerase Chain Reaction, Staining

KD serum has higher levels of secreted BMP6. The plasma levels of BMP6 from eight non-KD febrile controls and eight KD patients were examined using an ELISA assay. * P < 0.03 vs. febrile controls

Journal: Cell & Bioscience

Article Title: Serum IP-10 and IL-17 from Kawasaki disease patients induce calcification-related genes and proteins in human coronary artery smooth muscle cells in vitro

doi: 10.1186/s13578-020-00400-8

Figure Lengend Snippet: KD serum has higher levels of secreted BMP6. The plasma levels of BMP6 from eight non-KD febrile controls and eight KD patients were examined using an ELISA assay. * P < 0.03 vs. febrile controls

Article Snippet: IP-10, IL-17, and BMP6 ELISA kit, BMP2-specifc rabbit polyclonal antibody, BMP4-specific mouse monoclonal antibody, and BMP6-specific goat polyclonal antibody were come from the Biocompare (San Francisco, CA).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay